Glossary
Net peptide content
The proportion of a lyophilised vial that is actual peptide, the rest being counter-ions and water. It is usually lower than HPLC purity.
Further reading
- Peptide price per mg: how to compare vials properly
Why price per vial misleads, how to calculate price per mg and net-peptide price, and how pack sizes change what you really pay.
- TFA vs acetate peptide salts: why the counter-ion matters
Synthetic peptides are supplied as salts. What trifluoroacetate and acetate counter-ions are, why they change net content and when the choice matters.
- Planning peptide inventory for a research project
How to work out total quantity, choose vial sizes, track batches and avoid waste when sourcing research peptides for a laboratory project.
- How to read a peptide Certificate of Analysis
What each line of a peptide CoA means, which checks matter most, and the red flags that suggest a document is decoration rather than evidence.
- Purity versus net peptide content: why 99% is not 99%
HPLC purity and net peptide content measure different things. Understand the gap, why vials contain counter-ions and water, and how it affects solution concentrations.
- How research peptides are made and tested
From solid-phase synthesis to preparative HPLC, freeze-drying and quality control: what happens between an amino acid sequence and a vial.